3UYX
Crystal structures of globular head of 2009 pandemic H1N1 hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.661, 75.052, 60.134 |
| Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
| Resolution | 19.740 - 1.800 |
| R-factor | 0.1668 |
| Rwork | 0.165 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.964 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.381 | |
| Number of reflections | 37337 | |
| Completeness [%] | 99.6 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 20% PEG 3350, 0.2M ammonium nitrate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
