3UYV

Crystal structure of a glycosylated ice-binding protein (LeIBP) from Arctic yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2011-06-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54180
Spacegroup name	P 65 2 2
Unit cell lengths	58.640, 58.640, 292.641
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.780 - 2.430
R-factor	0.23587
Rwork	0.233
R-free	0.28617
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uyu
RMSD bond length	0.018
RMSD bond angle	2.208
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.780	2.560
High resolution limit [Å]	2.430	2.430
Number of reflections	11149
Completeness [%]	93.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	0.1M imidazole, pH 8.5, 1. M potassium phosphate, 0.5M sodium phosphate, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 292K