3UYV
Crystal structure of a glycosylated ice-binding protein (LeIBP) from Arctic yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 58.640, 58.640, 292.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.780 - 2.430 |
| R-factor | 0.23587 |
| Rwork | 0.233 |
| R-free | 0.28617 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uyu |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.208 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.780 | 2.560 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 11149 | |
| Completeness [%] | 93.8 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1M imidazole, pH 8.5, 1. M potassium phosphate, 0.5M sodium phosphate, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
