3UYS
Crystal structure of apo human ck1d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.770, 85.160, 89.120 |
| Unit cell angles | 70.19, 73.86, 86.15 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.238 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.049 | 0.644 |
| Number of reflections | 64456 | |
| Completeness [%] | 96.1 | 93.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100 mM Citrate, 0.2 or 0.4 M Na2SO4, 15-20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.0 |
