3UYR
Structure of a monoclonal antibody complexed with its MHC-I antigen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.07500 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.524, 40.423, 59.265 |
Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
Resolution | 45.067 - 1.700 |
R-factor | 0.1941 |
Rwork | 0.192 |
R-free | 0.22560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.168 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.070 |
High resolution limit [Å] | 1.700 |
Number of reflections | 49813 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.1M LiSO4, 0.1M Tris pH 8.5, 30% (w/v) PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |