3UYK
Spinosyn Rhamnosyltransferase SpnG complexed with spinosyn aglycone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.021, 57.076, 68.100 |
| Unit cell angles | 81.98, 74.10, 86.07 |
Refinement procedure
| Resolution | 29.830 - 1.700 |
| R-factor | 0.19113 |
| Rwork | 0.189 |
| R-free | 0.23431 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tsa |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (version: 5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.830 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 78024 | |
| Completeness [%] | 96.9 | 91.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 298.15 | 18% w/v PEG3350, 12.5-13.5% w/v glucose, 1% v/v glycerol, 0.1 M magnesium formate, 0.1 M sodium cacodylate, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 298.15K |
