3UYC
Designed protein KE59 R8_2/7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.569, 65.466, 75.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.595 - 2.200 |
| R-factor | 0.21187 |
| Rwork | 0.209 |
| R-free | 0.27524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igs |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.349 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.595 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.063 | 0.406 |
| Number of reflections | 25219 | |
| <I/σ(I)> | 22.7 | 6.4 |
| Completeness [%] | 97.8 | 96.5 |
| Redundancy | 8.3 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 30% PEG2000 MME, 0.1 M potassium thiocyanate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
