3UY8
Designed protein KE59 R5_11/5F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.961, 102.727, 66.296 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.980 - 2.410 |
| R-factor | 0.21884 |
| Rwork | 0.215 |
| R-free | 0.29897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uyc |
| RMSD bond length | 0.037 |
| RMSD bond angle | 2.782 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.980 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.455 |
| Number of reflections | 23532 | |
| <I/σ(I)> | 28.8 | 2.7 |
| Completeness [%] | 98.3 | 87.4 |
| Redundancy | 7.1 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% PEG3350, 0.01 M magnesium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
