3UY7
Designed protein KE59 R1 7/10H with G130S mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9197 |
| Spacegroup name | P 31 |
| Unit cell lengths | 61.529, 61.529, 119.961 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.987 - 1.450 |
| R-factor | 0.16478 |
| Rwork | 0.163 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uyc |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.987 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.578 |
| Number of reflections | 90084 | |
| <I/σ(I)> | 23.5 | 2.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20% PEG3350, 0.2 M sodium sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
