3UY5

crystal structure of Eis from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	H 3 2
Unit cell lengths	174.574, 174.574, 122.827
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.841 - 2.500
R-factor	0.1845
Rwork	0.183
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.114
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.460
Number of reflections	26334

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	300mM ammonium citrate, 12% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K