3UXI
Crystal structure of L-rhamnose isomerase W38A mutant from Bacillus halodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.380, 164.794, 92.837 |
| Unit cell angles | 90.00, 117.02, 90.00 |
Refinement procedure
| Resolution | 45.870 - 2.730 |
| R-factor | 0.19039 |
| Rwork | 0.186 |
| R-free | 0.26680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.661 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.730 | 2.750 |
| Number of reflections | 90338 | |
| Completeness [%] | 79.0 | 82.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 283 | 10% (w/v) PEG 8000, 0.1M HEPES pH 6.0, 0.2M sodium acetate, VAPOR DIFFUSION, temperature 283K |
