3UXF
Structure of the fimbrial protein FimP from Actonomyces oris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.239, 176.587, 40.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.817 - 1.603 |
| R-factor | 0.1708 |
| Rwork | 0.170 |
| R-free | 0.19610 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.432 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.820 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 71226 | |
| <I/σ(I)> | 21.4 | 5.8 |
| Completeness [%] | 97.5 | 95.1 |
| Redundancy | 5.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 20% PEG4000, 50 mM calcium chloride, 100 mM sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
