3UX1
Structural Characterization of Adeno-Associated Virus Serotype 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 32 |
| Unit cell lengths | 250.680, 250.680, 632.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.800 |
| R-factor | 0.2841 |
| Rwork | 0.283 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2QAO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.155 | |
| Number of reflections | 1039725 | |
| <I/σ(I)> | 15.367 | 2.87 |
| Completeness [%] | 95.1 | |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | virus concentration of ~5 mg/ml in 30 mM HEPES, pH 7.3, with 30 mM MgCl, 100 mM NaCl, 200 mM (NH4)2SO4, 5% PEG6000, and 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
