3UWZ
Crystal structure of Staphylococcus aureus triosephosphate isomerase complexed with glycerol-2-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-26 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.022, 79.022, 174.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.660 - 2.500 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m9y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.979 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.017 | 2.630 |
| High resolution limit [Å] | 2.497 | 2.500 |
| Rmerge | 0.524 | |
| Number of reflections | 19971 | |
| <I/σ(I)> | 27.4 | 1.5 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 14 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6M TRISODIUM CITRATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
