3UWY
Crystal structure of triosephosphate isomerase from Methicillin resistant Staphylococcus Aureus at 2.4 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.624, 76.624, 174.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m9y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.039 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.172 | 2.530 |
| High resolution limit [Å] | 2.396 | 2.400 |
| Rmerge | 0.556 | |
| Number of reflections | 21249 | |
| <I/σ(I)> | 14.6 | 1.4 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4M SODIUM MALONATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
