3UVT
Crystal structure of the third catalytic domain of ERp46
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.106, 61.886, 71.002 |
| Unit cell angles | 90.00, 106.37, 90.00 |
Refinement procedure
| Resolution | 68.120 - 2.000 |
| R-factor | 0.20599 |
| Rwork | 0.204 |
| R-free | 0.24549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f8u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.120 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.394 |
| Number of reflections | 36285 | |
| <I/σ(I)> | 21 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris (pH 5.5), 25% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
