3UUM
Crystal Structure of N-terminal first spectrin repeat of utrophin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.740, 58.020, 91.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.870 - 2.000 |
| R-factor | 0.20066 |
| Rwork | 0.197 |
| R-free | 0.26811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.590 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.870 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.141 | 0.141 |
| Number of reflections | 14759 | |
| <I/σ(I)> | 7.6 | 7.6 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294.15 | 20% PEG 8000, 0.1M Na Cacodylate, 0.2M Magnesium Acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |
