3UUL
Crystal Structure of first N-terminal utrophin spectrin repeat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.000, 58.660, 91.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.630 - 1.950 |
| R-factor | 0.20021 |
| Rwork | 0.198 |
| R-free | 0.23545 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s35 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.236 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0101) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.920 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.085 | 0.406 |
| Number of reflections | 16320 | |
| <I/σ(I)> | 10.2 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 25% PEG 4000, 0.1M Tris HCl, 0.2M Magnesium Chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
