3UU0
Crystal structure of L-rhamnose isomerase from Bacillus halodurans in complex with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.584, 165.553, 92.471 |
| Unit cell angles | 90.00, 115.82, 90.00 |
Refinement procedure
| Resolution | 46.620 - 2.700 |
| R-factor | 0.18392 |
| Rwork | 0.180 |
| R-free | 0.25469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.778 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.800 |
| Number of reflections | 56522 | |
| Completeness [%] | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 283 | 10% (w/v) PEG 8000, 0.1M HEPES pH 6.0, 0.2M sodium acetate, VAPOR DIFFUSION, temperature 283K |
