3UTK
Structure of the pilotin of the type II secretion system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0718 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.700, 53.095, 98.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.762 - 1.650 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.24970 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.918 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.200 | 0.336 |
| Number of reflections | 32440 | |
| <I/σ(I)> | 6.9 | 2.6 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 7.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2M ammonium sulfate, 2% PEG 400, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
