3UT1
Crystal structure of the 3-MBT repeat domain of L3MBTL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9179 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.287, 70.619, 58.574 |
Unit cell angles | 90.00, 119.95, 90.00 |
Refinement procedure
Resolution | 44.761 - 2.050 |
R-factor | 0.224 |
Rwork | 0.222 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | based on unpublished model of L3MBTL1 itself based on molecular replacement with coordinates from PDB entry 1OYX |
RMSD bond length | 0.011 |
RMSD bond angle | 1.309 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
Rmerge | 0.130 | 0.057 | 0.859 |
Number of reflections | 20483 | ||
<I/σ(I)> | 6.1 | ||
Completeness [%] | 99.9 | 99.8 | 99.5 |
Redundancy | 5.4 | 5.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 291 | 27% PEG-3350, 0.1M ammonium sulfate, 0.2M cobaltous chloride, 0.1M Bis-tris, pH 5.5, vapor diffusion, temperature 291K |