3UT0

Crystal structure of exo-1,3/1,4-beta-glucanase (EXOP) from Pseudoalteromonas sp. BB1

Replaces:  3F93

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	113
Detector technology	CCD
Collection date	2009-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	84.908, 257.821, 85.605
Unit cell angles	90.00, 115.47, 90.00

Refinement procedure

Resolution	38.328 - 2.300
R-factor	0.2252
Rwork	0.223
R-free	0.27010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3usz 3f95
RMSD bond length	0.007
RMSD bond angle	1.037
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.405
Number of reflections	143421
<I/σ(I)>	8	2.1
Completeness [%]	97.9	95.9
Redundancy	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M HEPES-NAOH, PH7.5, 2% PEG 400, 2.0M AMMONIUM SULFATE, VAPOR DIFFUSION, HANGING DROP, temperature 291K