3USZ

Crystal structure of truncated exo-1,3/1,4-beta-glucanase (EXOP) from Pseudoalteromonas sp. BB1

Replaces:  3F94

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	113
Detector technology	IMAGE PLATE
Collection date	2007-03-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	68.054, 179.414, 177.177
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.416 - 2.100
R-factor	0.1653
Rwork	0.164
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ex1
RMSD bond length	0.009
RMSD bond angle	1.068
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.350	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	0.446
Number of reflections	62062
<I/σ(I)>	11.7	1.9
Completeness [%]	97.5	86.7
Redundancy	3.4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1M BIS TRIS PROPANE PH6.5, 20% PEG 3350, 0.2M K/NA TARTRATE, VAPOR DIFFUSION, HANGING DROP, temperature 291K