Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 |
| Unit cell lengths | 125.460, 125.460, 39.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.066 - 2.601 |
| R-factor | 0.2025 |
| Rwork | 0.200 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lkv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.981 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.066 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 11229 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.1M AMMONIUM SULFATE, 0.3M SODIUM FORMATE, 0.1M TRIS, 1% PGA, 7% PEG8000, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
