3UST

Structure of BmNPV ORF075 (p33)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	54.520, 80.563, 130.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.370 - 2.100
R-factor	0.18586
Rwork	0.183
R-free	0.24079
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.371
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.370	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.057
Number of reflections	16085
Completeness [%]	98.6	97.8
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 3350, Proline, 1,6-hexanediol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 3350, Proline, 1,6-hexanediol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K