3USJ
Crystal structure of LeuT bound to L-leucine in space group P21 from lipid bicelles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.307, 179.880, 57.207 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 48.330 - 3.500 |
| R-factor | 0.2708 |
| Rwork | 0.264 |
| R-free | 0.30950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.609 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.140 | 0.340 |
| Number of reflections | 13846 | |
| <I/σ(I)> | 6.1 | 2.1 |
| Completeness [%] | 94.3 | 83.4 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM NaAc, 35% MPD, 5-10% PEG400, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
