3US5

Crystal structure of a RNA-binding domain of a poly-U binding splicing factor 60KDa (PUF60) from Homo sapiens at 1.38 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-15
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	43.433, 48.974, 56.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.950 - 1.380
R-factor	0.1444
Rwork	0.142
R-free	0.18130
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.499
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.950	36.950	1.450
High resolution limit [Å]	1.380	4.360	1.380
Rmerge		0.011	0.011
Total number of observations		5902	25286
Number of reflections	24223
<I/σ(I)>	9.3	12.5	0.6
Completeness [%]	95.4	99.8	100
Redundancy	6.9	6.5	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	2.40M ammonium sulfate, 0.1M TRIS pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K