3URM
Crystal structure of the periplasmic sugar binding protein ChvE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 65 |
Unit cell lengths | 130.076, 130.076, 64.676 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.577 - 1.801 |
R-factor | 0.1514 |
Rwork | 0.149 |
R-free | 0.19490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1drk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.891 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
<I/σ(I)> | 34.55 | 3.2 |
Completeness [%] | 87.0 | 96.9 |
Redundancy | 5.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 289 | 32% PEG4000, 0.2M Magnesium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |