3URG
The crystal structure of Anabaena CcbP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9790 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 50.740, 50.740, 150.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.003 |
R-factor | 0.2106 |
Rwork | 0.207 |
R-free | 0.24170 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.007 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.080 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 15726 | |
Completeness [%] | 98.3 | 91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 3M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 3M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |