3UR3

Structure of the Cmr2 subunit of the CRISPR RNA silencing complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2010-07-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97921
Spacegroup name	P 21 21 21
Unit cell lengths	70.635, 80.211, 143.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.681 - 2.405
R-factor	0.2222
Rwork	0.218
R-free	0.27340
RMSD bond length	0.013
RMSD bond angle	1.068
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	29299
Completeness [%]	90.7	65.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	303.15	0.2M CaCl2, 28% PEG 400, 0.1M HEPES Sodium, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303.15K