3UQ7
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) mutant L240S F247L (L9S F16L) in presence of 10 mM cysteamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.380, 266.450, 110.910 |
| Unit cell angles | 90.00, 109.47, 90.00 |
Refinement procedure
| Resolution | 19.966 - 3.800 |
| R-factor | 0.2164 |
| Rwork | 0.215 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vl0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.570 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_897) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 133.225 | 3.900 | |
| High resolution limit [Å] | 3.800 | 16.990 | 3.800 |
| Rmerge | 0.137 | 0.028 | 0.691 |
| Number of reflections | 56196 | 251 | 4189 |
| <I/σ(I)> | 11.83 | 45.56 | 2.94 |
| Completeness [%] | 99.1 | 37.7 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 10-12% PEG4000, 200 mM ammonium sulfate, 50 mM N-(2-acetamido)iminodiacetic acid (ADA), pH 6.5-6.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
