3UQ3
TPR2AB-domain:pHSP90-complex of yeast Sti1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-14 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.191, 62.897, 66.842 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.22027 |
| Rwork | 0.217 |
| R-free | 0.27854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1elr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.979 |
| Data reduction software | PROTEUM2 |
| Data scaling software | PROTEUM2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.082 | 0.352 |
| Number of reflections | 17924 | |
| <I/σ(I)> | 15.26 | 3.2 |
| Completeness [%] | 99.0 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 0.2 M TMAO, 0.1 M Tris, 20% PEG 2000 MME, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
