3UPV
TPR2B-domain:pHsp70-complex of yeast Sti1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 38.630, 38.630, 176.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.860 - 1.600 |
| R-factor | 0.18618 |
| Rwork | 0.183 |
| R-free | 0.25443 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1elr |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.884 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.063 | 0.215 |
| Number of reflections | 17394 | |
| <I/σ(I)> | 15.03 | 3.1 |
| Completeness [%] | 93.5 | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M LiCl, 2.2 M Ammoniumsulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
