3UP5
Crystal Structure of OTEMO complex with FAD and NADP (form 4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-20 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.837, 94.394, 93.135 |
| Unit cell angles | 90.00, 102.38, 90.00 |
Refinement procedure
| Resolution | 48.353 - 2.453 |
| R-factor | 0.1874 |
| Rwork | 0.185 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uoy |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.842 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.968 | 90.968 | 2.540 |
| High resolution limit [Å] | 2.453 | 5.280 | 2.453 |
| Rmerge | 0.097 | 0.048 | 0.349 |
| Number of reflections | 39964 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 96.6 | 91.5 | 98.2 |
| Redundancy | 2.7 | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 22% PEG3350, 0.1 M sodium/potassium phosphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
