3UOY
Crystal Structure of OTEMO complex with FAD and NADP (form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.753, 94.994, 93.089 |
| Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
| Resolution | 43.106 - 2.000 |
| R-factor | 0.1987 |
| Rwork | 0.196 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uov |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.264 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.969 | 90.969 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.109 | 0.038 | 0.478 |
| Number of reflections | 76439 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 99.7 | 98.5 | 100 |
| Redundancy | 3.6 | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 22% PEG3350, 0.1 M sodium/potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
