3UOT
Crystal Structure of MDC1 FHA Domain in Complex with a Phosphorylated Peptide from the MDC1 N-terminus
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.961 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.485, 59.880, 72.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.20687 |
| Rwork | 0.205 |
| R-free | 0.24262 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.037 | 0.315 |
| Number of reflections | 24225 | |
| Completeness [%] | 93.4 | 55.3 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 291 | 25% v/v ethanol, 0.1M sodium acetate pH 5.5, Microbatch, temperature 291K |
