3UO8
Crystal structure of the MALT1 paracaspase (P1 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.235, 61.158, 71.126 |
| Unit cell angles | 108.59, 97.23, 117.23 |
Refinement procedure
| Resolution | 34.262 - 1.900 |
| R-factor | 0.2089 |
| Rwork | 0.207 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.991 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.051 | 0.029 | 0.492 |
| Number of reflections | 63071 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 97.3 | 94.3 | 96.7 |
| Redundancy | 2.7 | 2.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES (pH 6.0), 0.2M calcium acetate, 2% benzamidine, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
