3UO1
Structure of a monoclonal antibody complexed with its MHC-I antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07500 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 192.583, 40.291, 59.353 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | ? - 1.641 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.930 |
| High resolution limit [Å] | 1.640 |
| Number of reflections | 54890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | antibody at 6.8 mg/ml mixed with equimolar peptide and then 1:1 with 0.1 M LiSO4, 0.1 M TRIS, 30% w/v PEG 3000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
