3UNZ

Aurora A in Complex with RPM1679

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	93
Detector technology	CCD
Collection date	2011-05-10
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 32
Unit cell lengths	85.780, 85.780, 76.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.885 - 2.800
Rwork	0.231
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fdn
RMSD bond length	0.007
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.078	0.397
Number of reflections	15425
<I/σ(I)>	27	4.6
Completeness [%]	99.4	99.4
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	10 mg/mL AURORA A protein, 1 mM RPM1679, 10 % (v/v) PEG 3350, 25 mM phosphate(Na/K pH 7.4), 5 % Tacismate pH 7.0 VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291K