3UNT
tRNA-guanine transglycosylase E339Q mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.290, 64.890, 70.238 |
| Unit cell angles | 90.00, 96.01, 90.00 |
Refinement procedure
| Resolution | 18.917 - 1.801 |
| R-factor | 0.1552 |
| Rwork | 0.154 |
| R-free | 0.18470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pud |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 36234 | |
| <I/σ(I)> | 10.9 | 2.8 |
| Completeness [%] | 95.7 | 87.5 |
| Redundancy | 2.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | TRIS 0.1M, DMSO 10% v/v, PEG8000 7% w/w, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
