3UNP
Structure of human SUN2 SUN domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 78.918, 78.918, 197.918 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.470 - 2.390 |
| R-factor | 0.22331 |
| Rwork | 0.218 |
| R-free | 0.26640 |
| Structure solution method | MIR |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.668 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.390 | 2.850 |
| Number of reflections | 11047 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 0.1M imidazole, 1.0M sodium acetate, 10 mM YCl3, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
