3UNG

Structure of the Cmr2 subunit of the CRISPR RNA silencing complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	93
Detector technology	CCD
Collection date	2011-07-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	73.167, 85.070, 140.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.614 - 2.310
R-factor	0.2064
Rwork	0.203
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.255
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	35291
Completeness [%]	90.7	51.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	303.15	0.2M CaCl2, 28% PEG 400, 0.1M HEPES Sodium, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303.15K