3UN9
Crystal structure of an immune receptor
Experimental procedure
| Experimental method | Platinum |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2010-01-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0719, 1.0716, 0.8550 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.011, 123.469, 145.848 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.240 - 2.650 |
| R-factor | 0.22566 |
| Rwork | 0.223 |
| R-free | 0.26711 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.568 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.240 | 2.719 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.282 | |
| Number of reflections | 25742 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 97.9 | 97.85 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 296 | 18% PEG 1000 and 200 mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 296.0K |
