3UMT
scFv12, Anti-BclA antibody single chain variable fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-10 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 65.290, 65.290, 96.202 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.914 - 1.798 |
R-factor | 0.1772 |
Rwork | 0.175 |
R-free | 0.22320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | VH domain from structure 1KTR VL domain from structure 1FGV |
RMSD bond length | 0.016 |
RMSD bond angle | 1.627 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.830 |
High resolution limit [Å] | 1.798 | 1.798 |
Rmerge | 0.040 | 0.341 |
Number of reflections | 22608 | |
<I/σ(I)> | 60 | 6.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 12 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 1M Sodium citrate, 0.1 M CHES pH9.5 diluted by water 4:1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |