3UMP
Crystal structure of the Phosphofructokinase-2 from Escherichia coli in complex with Cesium and ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 43.856, 88.914, 176.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.046 - 1.849 |
| R-factor | 0.1739 |
| Rwork | 0.172 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.960 |
| High resolution limit [Å] | 1.849 | 5.520 | 1.849 |
| Rmerge | 0.052 | 0.035 | 0.460 |
| Number of reflections | 109841 | 3983 | 17693 |
| <I/σ(I)> | 10.48 | 25.24 | 2.07 |
| Completeness [%] | 96.4 | 93.3 | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 291 | 23% PEG 4000, 0.1 M SODIUM ACETATE PH 4.75, 0.2 M AMMONIUM ACETATE, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
