3UL0
Mouse importin alpha: mouse CBP80Y8D cNLS complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-16 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.691, 89.898, 99.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.983 - 2.000 |
| R-factor | 0.1886 |
| Rwork | 0.188 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pjn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.966 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.983 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 48448 | |
| Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.7M sodium citrate, 10mM DTT, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
