3UKT
Structure of the C-linker/CNBHD of zELK channels in P1 21 1 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.660, 107.810, 77.580 |
| Unit cell angles | 90.00, 97.38, 90.00 |
Refinement procedure
| Resolution | 44.148 - 2.300 |
| R-factor | 0.2074 |
| Rwork | 0.205 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Structure of the corresponding selenomethionine derivative crystal |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.072 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.940 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.041 | 0.200 |
| Number of reflections | 37558 | |
| <I/σ(I)> | 12.5 | 2.5 |
| Completeness [%] | 93.1 | 66.3 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 6% (w/v) D-(+)-galactose, 180 mM ammonium sulfate, 22.5% (w/v) PEG 3350, 90 mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
