3UJO
Galactose-specific seed lectin from Dolichos lablab in complex with adenine and galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 32 |
| Unit cell lengths | 89.780, 89.780, 123.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.200 - 2.000 |
| R-factor | 0.20966 |
| Rwork | 0.207 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bjq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.915 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.920 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.106 | 0.520 |
| Number of reflections | 75331 | |
| <I/σ(I)> | 7.8 | 2.4 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 6.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 30% PEG 4K, 100mM Tris pH 8.5, 200mM Na-acetate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
