3UJL

Crystal structure of abscisic acid bound PYL2 in complex with type 2C protein phosphatase ABI2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-15
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	61.430, 98.587, 132.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.925 - 2.500
R-factor	0.2244
Rwork	0.223
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.406
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	23335
Completeness [%]	92.5	87.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	0.1 M HEPES, 6% (w/v) PEG 8000, 10% (w/v) sucrose, pH 7.0, VAPOR DIFFUSION, temperature 298K