3UIV
Human serum albumin-myristate-amantadine hydrochloride complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-28 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.270, 93.516, 96.217 |
| Unit cell angles | 74.91, 89.75, 80.04 |
Refinement procedure
| Resolution | 37.389 - 2.200 |
| R-factor | 0.2295 |
| Rwork | 0.226 |
| R-free | 0.29020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 66700 | |
| Completeness [%] | 95.0 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 32% (w/v) polyethylene glycol 3350, 50mM potassium phosphate (pH 7.5), 5% glycerol, 3% DMSO, 2% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
