3UIH
crystal structure of human Survivin in complex with Smac/DIABLO(1-15) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.29 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.246, 71.308, 81.095 |
| Unit cell angles | 90.00, 127.49, 90.00 |
Refinement procedure
| Resolution | 27.821 - 2.400 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f3h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.059 | 0.595 |
| Number of reflections | 20178 | |
| <I/σ(I)> | 22.9 | 2.6 |
| Completeness [%] | 98.4 | 90 |
| Redundancy | 7.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2 M potassium thiocyanate, 10% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
